Martin L, Bäurle A, Fiehn W, Volkmann M
Medizinische Klinik und Poliklinik, Universität Heidelberg, Germany.
Clin Lab. 2000;46(7-8):339-44.
"Soluble liver antigen" (SLA) has been reported to be an infrequent but in certain cases a unique marker of autoimmune hepatitis, with cytokeratins 8 and 18 as major antigenic components. Using precharacterized sera, we could confirm trypsin sensitivity and a molecular weight of approximately 50 kD of the reactive protein. However, the reaction differed from that of cytokeratins 8 and 18 by molecular weight and a pI of 7.5. A significant reactivity to cytokeratin 8 and 18 preparations was seen in only 1/12 patients. Immunoscreening of a human liver expression gene bank yielded no clones with sequence homology to cytokeratins. We conclude that reactivity in SLA positive sera is not mainly directed against cytokeratins 8/18 and recommend native antigen preparations for diagnostic use until the exact molecular nature of the 52 kD SLA antigen has been elucidated.
“可溶性肝抗原”（SLA）据报道是自身免疫性肝炎中一种罕见但在某些情况下独特的标志物，细胞角蛋白8和18是其主要抗原成分。使用预先鉴定的血清，我们能够证实反应性蛋白对胰蛋白酶敏感且分子量约为50kD。然而，该反应在分子量和pI为7.5方面与细胞角蛋白8和18不同。仅1/12的患者对细胞角蛋白8和18制剂有显著反应性。对人肝表达基因库进行免疫筛选未得到与细胞角蛋白具有序列同源性的克隆。我们得出结论，SLA阳性血清中的反应性并非主要针对细胞角蛋白8/18，并建议在52kD SLA抗原的确切分子性质阐明之前，使用天然抗原制剂进行诊断。
