慢生根瘤菌中低吲哚-3-乙酸产生突变体的分离与鉴定
Isolation and characterization of low-indole-3-acetic acid-producing mutants from Bradyrhizobium elkanii.
作者信息
Yagi K, Matsumoto T, Chujo T, Nojiri H, Omori T, Minamisawa K, Nishiyama M, Yamane H
机构信息
Biotechnology Research Center, The University of Tokyo, Japan.
出版信息
Biosci Biotechnol Biochem. 2000 Jul;64(7):1359-64. doi: 10.1271/bbb.64.1359.
PMID:10945251
Abstract
We isolated 11 low-indole-3-acetic acid (IAA)-producing mutants of Bradyrhizobium elkanii by Tn5 mutagenesis. The amount of IAA produced by each mutant was 2.2-13.6% of that of the wild-type. It was found by resting cell reactions that the biosynthetic step to convert indole-3-pyruvic acid to indole-3-acetaldehyde was blocked in all the mutants.
摘要
我们通过Tn5诱变分离出11株慢生根瘤菌低吲哚-3-乙酸（IAA）产生突变体。每个突变体产生的IAA量为野生型的2.2%-13.6%。通过静息细胞反应发现，所有突变体中吲哚-3-丙酮酸转化为吲哚-3-乙醛的生物合成步骤均被阻断。
Zotero 插件