富含组氨酸糖蛋白与6-氨基己酸对体外纤溶酶生成及纤维蛋白溶解作用的比较

Comparison of the effect of histidine-rich glycoprotein and 6-aminohexanoic acid on plasmin production and fibrinolysis in vitro.

作者信息

Horne M K, Goad J L, Merryman P K, Cullinane A M

机构信息

Hematology Service, Clinical Pathology Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA.

出版信息

Thromb Res. 2000 Jul 15;99(2):179-86. doi: 10.1016/s0049-3848(00)00231-0.

DOI:10.1016/s0049-3848(00)00231-0

PMID:10946092

Abstract

Because histidine-rich glycoprotein binds to the kringle 1-3 domain of plasminogen, it may affect fibrinolysis by reducing fibrin-dependent plasmin production, and in this way it could be mechanistically analogous to 6-aminohexanoic acid. We tested this hypothesis by comparing the effects of histidine-rich glycoprotein and 6-aminohexanoic acid in an in vitro assay of fibrin-dependent plasmin production mediated by tissue plasminogen activator. Whereas 1 mM of 6-aminohexanoic acid increased the K(m) of the reaction from approximately 0.22 microM to approximately 1.7 microM, 2 microM of histidine-rich glycoprotein had no discernible effect. Similar results were obtained in an assay based upon fibrin clot lysis. Therefore, we could not document an effect of histidine-rich glycoprotein on the rate of fibrin-dependent plasmin production.

摘要

由于富含组氨酸的糖蛋白与纤溶酶原的kringle 1-3结构域结合，它可能通过减少纤维蛋白依赖性纤溶酶的产生来影响纤维蛋白溶解，从这个角度来看，其作用机制可能类似于6-氨基己酸。我们通过在由组织纤溶酶原激活剂介导的纤维蛋白依赖性纤溶酶产生的体外试验中比较富含组氨酸的糖蛋白和6-氨基己酸的作用，来验证这一假设。1 mM的6-氨基己酸可使反应的米氏常数（K(m)）从约0.22 microM增加到约1.7 microM，而2 microM的富含组氨酸的糖蛋白则没有明显作用。在基于纤维蛋白凝块溶解的试验中也得到了类似结果。因此，我们无法证明富含组氨酸的糖蛋白对纤维蛋白依赖性纤溶酶产生速率有影响。