Pusch W, Balvers M, Weinbauer G F, Ivell R
Institute of Anatomy, University Hospital, Hamburg-Eppendorf, 20246 Hamburg, Germany. Institute for Hormone and Fertility Research, University of Hamburg, 22529 Hamburg, Germany.
Biol Reprod. 2000 Sep;63(3):763-8. doi: 10.1095/biolreprod63.3.763.
The structure of the endozepine-like peptide (ELP) gene is closely related to the intracellular acyl-CoA binding protein (ACBP), but unlike the generalized distribution of the latter, it is restricted to the male germ cells of the testis. In the present study, a combination of nonradioactive in situ mRNA hybridization and immunohistochemistry was used to precisely determine the cellular expression patterns of ELP mRNA and protein in control and methoxyacetic acid (MAA)-treated rat testes. ELP transcripts are first detectable in late stages (step 6) of round spermatids, with transcription increasing through late-elongating steps. Translation of the ELP mRNA is delayed, with first immunohistochemical staining occurring in elongated spermatids at step 16, and protein accumulating through step 19. ELP immunoreactivity proves to be an excellent marker for late spermatid stages and highlights the presumably clonal recovery of spermatids following MAA treatment.
内源性类阿片样肽（ELP）基因的结构与细胞内酰基辅酶A结合蛋白（ACBP）密切相关，但与后者的广泛分布不同，它仅限于睾丸的雄性生殖细胞。在本研究中，采用非放射性原位mRNA杂交和免疫组织化学相结合的方法，精确测定对照和甲氧基乙酸（MAA）处理的大鼠睾丸中ELP mRNA和蛋白的细胞表达模式。ELP转录本首先在圆形精子细胞的后期阶段（第6步）中可检测到，转录在晚期伸长阶段增加。ELP mRNA的翻译延迟，首次免疫组织化学染色出现在第16步的伸长精子细胞中，并且蛋白在第19步积累。ELP免疫反应性被证明是晚期精子细胞阶段的一个优秀标记，并突出了MAA处理后精子细胞可能的克隆性恢复。
