Balan I S, Ugrumov M V, Calas A, Mailly P, Krieger M, Thibault J
Laboratory of Neurohistology, Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow 117808, Russia.
J Comp Neurol. 2000 Sep 18;425(2):167-76. doi: 10.1002/1096-9861(20000918)425:2<167::aid-cne1>3.0.co;2-k.
In this quantitative and semiquantitative immunocytochemical study, the authors evaluated the differentiation of neurons expressing tyrosine hydroxylase (TH) and/or aromatic L-amino acid decarboxylase (AADC) in the mediobasal hypothalamus (MBH) of male and female rats on embryonic day 18 (E18), E20, and postnatal day 9 (P9). Four neuronal populations were distinguished according to either enzyme expression or neuron location. The earliest and most prominent first population was represented by TH-immunoreactive (IR)/AADC-immunonegative (IN) neurons that were detected initially at E18 and always were located in the ventrolateral region of the MBH. The second population of TH-IN/AADC-IR neurons was observed first at E20 and, after that time, was distributed dorsomedially. The third minor population of TH-IR/AADC-IR neurons initially was detected at E20 and was located dorsomedially. The fourth population was represented by TH-IR/AADC-IN neurons that were distributed in the dorsomedial region at any studied age. The numbers of TH-IR and AADC-IR neurons increased from their initial detection at E18 and E20 until P9. The area of TH-IR and AADC-IR neurons also increased from E18 to E20 and from E20 to P9, respectively. Both TH-IR and AADC-IR neurons showed sex differences in the neuron number, size, and optic density (OD). The numbers of TH-IR neurons in males exceeded those of females at E20 and at P9, although, at P9, sexual dimorphism was a characteristic only of the ventrolateral population. The area and OD of TH-IR neurons from females exceeded those from males in the entire mediobasal hypothalamus (MBH) at E18 and E20 but only in its dorsomedial region at P9. Sexual dimorphism also was an attribute of AADC-IR neurons at E20 and P9. Their number, size, and OD were significantly higher in females than in males. Thus, the MBH of perinatal rats contained two major populations of TH-IR/AADC-IN or TH-IN-AADC-IR neurons and a minor population of TH-IR/AADC-IR neurons. The differentiating neurons expressing either enzyme showed sexual dimorphism.
在这项定量和半定量免疫细胞化学研究中，作者评估了胚胎第18天（E18）、E20和出生后第9天（P9）的雄性和雌性大鼠中脑基底部（MBH）中表达酪氨酸羟化酶（TH）和/或芳香族L-氨基酸脱羧酶（AADC）的神经元的分化情况。根据酶表达或神经元位置区分出四个神经元群体。最早且最显著的第一个群体由TH免疫反应性（IR）/AADC免疫阴性（IN）神经元代表，最初在E18时检测到，且始终位于MBH的腹外侧区域。第二群体的TH-IN/AADC-IR神经元最早在E20时观察到，此后分布于背内侧。第三小群体的TH-IR/AADC-IR神经元最初在E20时检测到，位于背内侧。第四群体由TH-IR/AADC-IN神经元代表，在任何研究年龄均分布于背内侧区域。TH-IR和AADC-IR神经元的数量从E18和E2时最初检测到开始增加，直至P9。TH-IR和AADC-IR神经元的面积也分别从E18增加到E20以及从E20增加到P9。TH-IR和AADC-IR神经元在神经元数量、大小和光密度（OD）方面均表现出性别差异。在E20和P9时，雄性TH-IR神经元的数量超过雌性，不过在P9时，性二态性仅为腹外侧群体的特征。在E18和E20时，雌性TH-IR神经元的面积和OD超过雄性，且在整个中脑基底部（MBH）均如此，但在P9时仅在其背内侧区域如此。性二态性也是E20和P9时AADC-IR神经元的一个特征。雌性的数量、大小和OD显著高于雄性。因此，围产期大鼠的MBH包含两个主要群体的TH-IR/AADC-IN或TH-IN-AADC-IR神经元以及一个小群体的TH-IR/AADC-IR神经元。表达这两种酶之一的分化神经元表现出性二态性。
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