韩国猪繁殖与呼吸综合征病毒分离株开放阅读框5基因的限制性片段长度多态性分析
Restriction fragment length polymorphism analysis of open reading frame 5 gene of porcine reproductive and respiratory syndrome virus isolates in Korea.
作者信息
Cheon D S, Chae C
机构信息
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon, Republic of Korea.
出版信息
Arch Virol. 2000;145(7):1481-8. doi: 10.1007/s007050070105.
The genetic variability of porcine reproductive and respiratory syndrome virus (PRRSV) was studied by restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR)-amplified fragments among 50 Korean isolates from open reading frame 5. All Korean PRRSVs were isolated from the field cases after the marketing of an U.S. ATCC VR2332-derived modified live PRRSV vaccine. Combining the restriction enzyme digestion patterns obtained with MluI, HincII, SacII, and HaeIII, we observed 19 distinct RFLP patterns. Seventeen out of 50 PRRSV isolates (34%) exhibited the modified live PRRSV vaccine RFLP pattern. The genomic variations that have been identified in the present study seemed to represent characteristic features of the Korean PRRSV isolates. PCR-based RFLP analysis using several restriction enzymes provides a good genetic estimate for isolate differentiation.
通过聚合酶链反应（PCR）扩增的来自开放阅读框5的片段的限制性片段长度多态性（RFLP），对50株韩国猪繁殖与呼吸综合征病毒（PRRSV）分离株的遗传变异性进行了研究。所有韩国PRRSV均从美国ATCC VR2332衍生的改良活PRRSV疫苗上市后的田间病例中分离得到。结合用MluI、HincII、SacII和HaeIII获得的限制性酶切图谱，我们观察到19种不同的RFLP模式。50株PRRSV分离株中有17株（34%）呈现改良活PRRSV疫苗的RFLP模式。本研究中鉴定出的基因组变异似乎代表了韩国PRRSV分离株的特征。使用几种限制性酶进行基于PCR的RFLP分析为分离株的鉴别提供了良好的遗传评估。
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