一种用于定量疑似克雅氏病患者脑脊液中14-3-3蛋白的酶联免疫吸附测定法。
An enzyme-linked immunosorbent assay to quantify 14-3-3 proteins in the cerebrospinal fluid of suspected Creutzfeldt-Jakob disease patients.
作者信息
Kenney K, Brechtel C, Takahashi H, Kurohara K, Anderson P, Gibbs C J
机构信息
Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892-4122, USA.
出版信息
Ann Neurol. 2000 Sep;48(3):395-8.
The detection of 14-3-3 protein by Western immunoblot is a sensitive and specific cerebrospinal fluid marker of Creutzfeldt-Jakob disease (CJD). We developed a quantitative enzyme-linked immunosorbent assay (ELISA) that reliably detects 14-3-3 in cerebrospinal fluid. In a prospective study of 147 cerebrospinal fluid samples, the mean 14-3-3 concentration among pathologically confirmed CJD patients (28.0+/-20.6 ng/ml, n = 41) is significantly higher than the mean in the cerebrospinal fluid of those with other neurological disorders (3.1+/-2.9 ng/ ml, n = 84). At a cutoff value of 8.3 ng/ml, the ELISA has a sensitivity of 92.7% and a specificity of 97.6%. The 14-3-3 ELISA supports a diagnosis of CJD in patients who fulfill clinical criteria for possible CJD.
通过蛋白质免疫印迹法检测14-3-3蛋白是克雅氏病（CJD）敏感且特异的脑脊液标志物。我们开发了一种定量酶联免疫吸附测定法（ELISA），可可靠地检测脑脊液中的14-3-3。在一项对147份脑脊液样本的前瞻性研究中，经病理确诊的CJD患者脑脊液中14-3-3的平均浓度（28.0±20.6 ng/ml，n = 41）显著高于其他神经系统疾病患者脑脊液中的平均浓度（3.1±2.9 ng/ml，n = 84）。在截断值为8.3 ng/ml时，ELISA的灵敏度为92.7%，特异性为97.6%。14-3-3 ELISA有助于对符合可能CJD临床标准的患者进行CJD诊断。
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