通过12S rRNA基因片段的聚合酶链反应-限制性片段长度多态性鉴定尼罗尖吻鲈（Lates niloticus）、石斑鱼（Epinephelus guaza）和多锯鲈（Polyprion americanus）。

Identification of Nile perch (Lates niloticus), grouper (Epinephelus guaza), and wreck fish (Polyprion americanus) by polymerase chain reaction-restriction fragment length polymorphism of a 12S rRNA gene fragment.

作者信息

Asensio L, González I, Fernández A, Céspedes A, Hernández P E, García T, Martín R

机构信息

Departamento de Nutrición y Bromatología III (Higiene y Tecnología de los Alimentos), Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.

出版信息

J Food Prot. 2000 Sep;63(9):1248-52. doi: 10.4315/0362-028x-63.9.1248.

DOI:10.4315/0362-028x-63.9.1248

PMID:10983800

Abstract

Restriction site analysis of polymerase chain reaction (PCR) products from a conserved region of the 12S rRNA gene has been used for the specific identification of Nile perch (Lates niloticus), grouper (Epinephelus guaza), and wreck fish (Polyprion americanus). Amplification of DNA isolated from muscle samples was carried out using a set of primers flanking a region of 436 bp from the mitochondrial 12S rRNA gene. Digestions of the PCR products with RsaI and Sau96I endonucleases, followed by agarose gel electrophoresis of the digested PCR products, yielded specific profiles that enabled direct identification of each species analyzed.

摘要

对来自12S rRNA基因保守区域的聚合酶链反应（PCR）产物进行限制性酶切位点分析，已被用于尼罗尖吻鲈（Lates niloticus）、石斑鱼（Epinephelus guaza）和多锯鲈（Polyprion americanus）的特异性鉴定。使用一组侧翼为线粒体12S rRNA基因436 bp区域的引物，对从肌肉样本中分离的DNA进行扩增。用RsaI和Sau96I内切酶消化PCR产物，然后对消化后的PCR产物进行琼脂糖凝胶电泳，得到的特异性图谱能够直接鉴定所分析的每个物种。