Song K, Jung D, Jung Y, Lee S G, Lee I
Department of Biochemistry, University of Ulsan College of Medicine, Seoul, South Korea.
FEBS Lett. 2000 Sep 8;481(1):81-5. doi: 10.1016/s0014-5793(00)01958-x.
Ku, a heterodimer of 70- and 80-kDa subunits, plays a general role in the metabolism of DNA ends in eukaryotic cells, including double-strand DNA break repair, V(D)J recombination, and maintenance of telomeres. We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80. Two reactive clones were found to encode the dimerization domain of TRF2, a mammalian telomeric protein that binds to duplex TTAGGG repeats at chromosome ends. This interaction was confirmed using bacterial fusion proteins and co-immunoprecipitations from eukaryotic cells overexpressing TRF2. The transfected TFR2 colocalized with Ku70.
Ku是由70 kDa和80 kDa亚基组成的异二聚体，在真核细胞DNA末端代谢中发挥一般作用，包括双链DNA断裂修复、V(D)J重组和端粒维持。我们利用酵母双杂交系统来鉴定除Ku80之外与Ku70相互作用的蛋白质。发现两个反应性克隆编码TRF2的二聚化结构域，TRF2是一种哺乳动物端粒蛋白，可结合染色体末端的双链TTAGGG重复序列。使用细菌融合蛋白和来自过表达TRF2的真核细胞的共免疫沉淀法证实了这种相互作用。转染的TFR2与Ku70共定位。
