一种高灵敏度和特异性的丙型肝炎病毒抗体酶联免疫吸附测定法。
A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus.
作者信息
Eroglu C, Yildiz E, Ozturk M, Pinarbasi E
机构信息
Department of Molecular Biology and Genetics, Science Faculty, Bilkent University, Ankara, Turkey.
出版信息
Acta Virol. 2000 Feb;44(1):29-33.
In this study, a 178 amino acids long portion of the hepatitis C virus (HCV) core gene was cloned, sequenced, expressed in Escherichia coli, and purified. The resulting antigen (C178) was tested with human sera enzyme-linked immunosorbent assay (ELISA) in order to assess its ability to diagnose HCV. It was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3rd generation enzyme immunoassay (EIA) as HCV-positive, had antibodies against the C178 antigen. This antigen gave no false positive results when tested with anti-HCV-negative sera.
在本研究中，克隆、测序了丙型肝炎病毒（HCV）核心基因一段长度为178个氨基酸的片段，在大肠杆菌中表达并纯化。用人类血清酶联免疫吸附测定法（ELISA）对所得抗原（C178）进行检测，以评估其诊断HCV的能力。ELISA结果显示，先前通过第三代酶免疫测定法（EIA）诊断为HCV阳性的患者血清中，92%含有针对C178抗原的抗体。用抗HCV阴性血清检测时，该抗原未出现假阳性结果。
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