中性锰离子依赖的脱氧核糖核酸酶定位的电子免疫组织化学分析。I. 铁蛋白和胶体金与抗中性锰离子依赖的脱氧核糖核酸酶单特异性抗体的缀合物的合成
[Electron immunohistochemical analysis of localization of neutral Mn2+-dependent DNAase. I. Synthesis of ferritin and colloidal gold conjugates with monospecific antibodies against neutral Mn2+-dependent DNAase].
作者信息
Abramova Z I, Zotkina N L, Vinter V G
机构信息
Laboratory of Biochemistry of Nucleic Acids, Kazan' State University.
出版信息
Tsitologiia. 2000;42(7):681-7.
PMID:10994086
Abstract
Rabbit antibodies against a neutral Mn(2+)-dependent rat liver DNAse were obtained, whose specificity towards DNAse was ascertained by suppression of the enzyme activity both in vitro system and immunoblotting assays. Procedures of synthesis of ferritin and colloidal gold conjugates with antibodies are described. The biological activity of the conjugates proved to be similar to that of the original antibodies.
摘要
获得了针对一种中性锰（2+）依赖性大鼠肝脏DNA酶的兔抗体，通过在体外系统和免疫印迹分析中抑制酶活性来确定其对DNA酶的特异性。描述了铁蛋白和胶体金与抗体的缀合物的合成过程。结果证明缀合物的生物活性与原始抗体相似。
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