关于5-甲基尿苷在大肠杆菌转移核糖核酸中功能的生理生化研究。
Physiological and biochemical studies on the function of 5-methyluridine in the transfer ribonucleic acid of Escherichia coli.
作者信息
Björk G R, Neidhardt F C
出版信息
J Bacteriol. 1975 Oct;124(1):99-111. doi: 10.1128/jb.124.1.99-111.1975.
Abstract
Matched pairs of transductant strains differing by the presence of absence of 5-methyluridine (ribothymidine) (m5U) in their transfer ribonucleic acid (tRNA) were used to study the function of this modified nucleoside in Escherichia coli. Ordinary measurements of growth rate in different media revealed no effect of the loss of m5U in tRNA. A gene located close to trmA (the structural cistron for the methyltransferase that produces m5U in tRNA), however, was found to reduce the growth rates significantly, depending on the medium and the temperature of cultivation. Measurement of codon recognition, macromolecular composition, tRNA binding to the ribosome, and the rate of protein chain elongation in vivo indicated no disadvantage caused by the lack of m5U. The regulation of ilv and his operons seemed also to be unaffected by the absence of m5U in the tRNA. In a mixed population experiment, however, cells possessing m5U in their tRNA seemed to have a distinct advantage over cells lacking this modified nucleoside. This experiment provides the first indication of the overall value of m5U in tRNA.
摘要
通过在其转移核糖核酸（tRNA）中存在或不存在5-甲基尿苷（核糖胸腺嘧啶核苷）（m5U）而不同的转导菌株配对，用于研究这种修饰核苷在大肠杆菌中的功能。在不同培养基中对生长速率的常规测量显示，tRNA中m5U的缺失没有影响。然而，发现一个位于trmA（在tRNA中产生m5U的甲基转移酶的结构顺反子）附近的基因，根据培养基和培养温度，会显著降低生长速率。对密码子识别、大分子组成、tRNA与核糖体的结合以及体内蛋白质链延伸速率的测量表明，缺乏m5U不会造成不利影响。ilv和his操纵子的调控似乎也不受tRNA中m5U缺失的影响。然而，在混合群体实验中，tRNA中含有m5U的细胞似乎比缺乏这种修饰核苷的细胞具有明显优势。该实验首次表明了tRNA中m5U的整体价值。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
ROLE OF VALYL-SRNA SYNTHETASE IN ENZYME REPRESSION.缬氨酰 - 转运核糖核酸合成酶在酶阻遏中的作用。
Proc Natl Acad Sci U S A. 1965 Mar;53(3):539-43. doi: 10.1073/pnas.53.3.539.
3
EVIDENCE FOR THE OCCURRENCE OF A COMMON PENTANUCLEOTIDE SEQUENCE IN THE STRUCTURES OF TRANSFER RIBONUCLEIC ACIDS.转运核糖核酸结构中存在常见五核苷酸序列的证据。
J Biol Chem. 1965 Mar;240:1267-73.
4
THE RELEASE OF RIBONUCLEASE INTO THE MEDIUM WHEN ESCHERICHIA COLI CELLS ARE CONVERTED TO SPEROPLASTS.大肠杆菌细胞转化为原生质体时核糖核酸酶释放到培养基中的情况。
J Biol Chem. 1964 Nov;239:3893-900.
5
The effect of enzymatically synthesized ribonucleic acid on amino acid incorporation by a soluble protein-ribosome system from Escherichia coli.酶促合成的核糖核酸对大肠杆菌可溶性蛋白质 - 核糖体系统掺入氨基酸的影响。
Proc Natl Acad Sci U S A. 1962 Jan 15;48(1):94-104. doi: 10.1073/pnas.48.1.94.
6
Chromosomal alterations affecting the regulation of histidine biosynthetic enzymes in Salmonella.影响沙门氏菌中组氨酸生物合成酶调控的染色体改变。
J Mol Biol. 1963 Jul;7:23-42. doi: 10.1016/s0022-2836(63)80016-9.
7
"Molecular sieving" of bacterial RNA.细菌RNA的“分子筛”作用
Arch Biochem Biophys. 1962 Sep;Suppl 1:276-82.
8
A genetic locus for the regulation of ribonucleic acid synthesis.一个用于调节核糖核酸合成的基因位点。
Proc Natl Acad Sci U S A. 1961 Dec 15;47(12):2005-14. doi: 10.1073/pnas.47.12.2005.
9
Use of chloramphenicol to study control of RNA synthesis in bacteria.使用氯霉素研究细菌中RNA合成的调控。
Biochim Biophys Acta. 1961 Oct 14;53:96-110. doi: 10.1016/0006-3002(61)90797-1.
10
The sequence of phenylalanine tRNA from E. coli.来自大肠杆菌的苯丙氨酸转运核糖核酸的序列。
FEBS Lett. 1969 Jun;3(4):275-278. doi: 10.1016/0014-5793(69)80157-2.
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