Dogusan Z, Book M L, Verdood P, Yu-Lee L Y, Hooghe-Peters E L
Department of Pharmacology, Medical School, Free University of Brussels (VUB), Laarbeeklaan, 103, B-1090 Brussels, Belgium.
Eur Cytokine Netw. 2000 Sep;11(3):435-42.
It has been proposed that prolactin (PRL) is a lympho-hemopoietic growth and differentiation factor. We show here by Western blotting that PRL-receptors (PRL-R) are expressed in normal rat bone marrow and spleen cells. We also show that PRL stimulates the phosphorylation of the PRL-R-associated Janus tyrosine kinase (JAK)-2 in rat bone marrow and spleen cells. This leads to the activation and subsequent binding of signal transducer and activator of transcription (Stat) 5b to an interferon regulatory factor-1 (IRF-1) gamma activation sequence (GAS) as visualized by electromobility shift assay. As shown after reverse transcription of mRNA by polymerase chain reaction, PRL, at physiological concentrations (0.01 microg/ml), stimulates the expression of the IRF-1 gene in these normal cells. PRL could thus affect several aspects of the immune response.
有人提出催乳素（PRL）是一种淋巴细胞生成和造血的生长及分化因子。我们在此通过蛋白质免疫印迹法表明，催乳素受体（PRL-R）在正常大鼠骨髓和脾细胞中表达。我们还表明，催乳素可刺激大鼠骨髓和脾细胞中与催乳素受体相关的Janus酪氨酸激酶（JAK）-2的磷酸化。通过电泳迁移率变动分析可见，这导致信号转导和转录激活因子（Stat）5b的激活以及随后与干扰素调节因子-1（IRF-1）γ激活序列（GAS）的结合。如通过聚合酶链反应对mRNA进行逆转录后所示，生理浓度（0.01微克/毫升）的催乳素可刺激这些正常细胞中IRF-1基因的表达。因此，催乳素可能会影响免疫反应的多个方面。
