O'Brochta D A, Atkinson P W, Lehane M J
Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, College Park, MD 20742-4450, USA.
Insect Mol Biol. 2000 Oct;9(5):531-8. doi: 10.1046/j.1365-2583.2000.00217.x.
The ability of the Hermes transposable element to function as a germ line transformation vector was tested in the stable fly, Stomoxys calcitrans. Plasmid-based transposable element mobility assays indicated moderate mobility of Hermes in this species. Germline transformants were created using a Hermes element containing the enhanced green fluorescent protein (EGFP) under the regulatory control of the promoter from Actin5C gene of Drosophila melanogaster. Approximately 4% of the fifty-five adults that developed from the 1903 G(0) embryos injected with the vector produced transgenic progeny. In the four transgenic lines established, the EGFP expression pattern was distinctly nonuniform and levels of expression were low. Promoters other than the one from the Actin5C gene of D. melanogaster should be considered for widespread, constitutive expression. All transgenic lines contained multiple (2-4) integrated Hermes elements. Hermes integration events occurred through a canonical cut-and-paste mechanism.
在厩螫蝇（Stomoxys calcitrans）这种稳定的果蝇中，测试了赫耳墨斯转座元件作为种系转化载体的功能。基于质粒的转座元件迁移分析表明，赫耳墨斯在该物种中的迁移率适中。利用含有在黑腹果蝇肌动蛋白5C基因启动子调控下的增强型绿色荧光蛋白（EGFP）的赫耳墨斯元件创建了种系转化体。从注射了载体的1903个G(0)胚胎发育而来的55只成虫中，约4%产生了转基因后代。在建立的四个转基因品系中，EGFP的表达模式明显不均匀，且表达水平较低。为实现广泛的组成型表达，应考虑使用除黑腹果蝇肌动蛋白5C基因启动子之外的其他启动子。所有转基因品系都含有多个（2 - 4个）整合的赫耳墨斯元件。赫耳墨斯的整合事件是通过典型的剪切粘贴机制发生的。
