使用4-N-二甲基氨基偶氮苯4'-异硫氰酸酯对多肽进行手动固相序列分析。

Manual solid phase sequence analysis of polypeptides using 4-N-N,-dimethylaminoazobenzene 4'-isothiocyanate.

作者信息

Chang J Y

出版信息

Biochim Biophys Acta. 1979 May 23;578(1):188-95. doi: 10.1016/0005-2795(79)90126-0.

Abstract

A manual solid-phase method for sequence analysis of polypeptides is described. The immobilized polypeptide was subjected to stepwise degradation by Edman-type reagent, using the 4-N,N-dimethylaminoazobenzene 4'-isothiocyanate phenyllisothiocyanate double coupling method. The N-terminal amino acids were released (after conversion reaction) as 4-N,N-dimethylaminoazobenzene 4'-thiohydantoin (identified by thin layer chromatography) and phenylthiohydantoin derivatives. The method required 2--10 nmol polypeptide.

摘要

本文描述了一种用于多肽序列分析的手动固相方法。使用4-N，N-二甲基氨基偶氮苯4'-异硫氰酸苯异硫氰酸酯双偶联法，使固定化多肽通过埃德曼型试剂进行逐步降解。N端氨基酸（经转化反应后）以4-N，N-二甲基氨基偶氮苯4'-硫代乙内酰脲（通过薄层色谱法鉴定）和苯硫代乙内酰脲衍生物的形式释放。该方法需要2-10 nmol的多肽。