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[在蒙得维的亚沙门氏菌中构建高频重组菌株(作者译)]

[Construction of Hfr strains in "Salmonella montevideo" (author's transl)].

作者信息

Dassa E

出版信息

Ann Microbiol (Paris). 1975 Apr;126(3):315-26.

PMID:1103675
Abstract

Lac+ variants of Salmonella montevideo were recovered from mating with an Escherichia coli strain harbouring the thermosensitive sex-factor Ft114-lac+. The properties of these variants suggest that this F' factor behaves identically in Salmonella and Escherichia. From these variants, it is possible to select mutants in which lac expression is stable at high temperature (42 degrees C) and it was proved that they behave as Hfr's. Twelve independently isolated Hfr's were similar with respect to both point of origin and sequence of markers transferred. These results suggest that our strain of S. montevideo carries an F-factor affinity site near met C gene.

摘要

从与携带温度敏感型性因子Ft114-lac +的大肠杆菌菌株交配中获得了蒙得维的亚沙门氏菌的Lac +变体。这些变体的特性表明,这种F'因子在沙门氏菌和大肠杆菌中的行为相同。从这些变体中,可以选择在高温(42摄氏度)下lac表达稳定的突变体,并且已证明它们表现为高频重组菌株(Hfr's)。十二个独立分离的Hfr's在起源点和转移标记的序列方面都相似。这些结果表明,我们的蒙得维的亚沙门氏菌菌株在met C基因附近携带一个F因子亲和位点。

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