Gionfriddo J R, Davidson H, Asem E K, Krohne S G
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47906, USA.
Am J Vet Res. 2000 Oct;61(10):1294-7. doi: 10.2460/ajvr.2000.61.1294.
To determine whether the tears of llamas, sheep, and cattle contain lysozyme and compare lysozyme concentrations in tears among these species.
40 llamas, 5 sheep, and 36 cattle.
Electrophoresis, western blot immunoassay for lysozyme, a spectrophotometric assay to detect tear lysozyme by its ability to lyse a suspension of Micrococcus lysodeiticus, and a microtiter plate colorometric assay were performed.
A 13.6-kd protein band was detected by use of electrophoresis and western blot immunoassay in llama and sheep tears but not cattle tears. Results of spectrophotometric assay suggested that llama and sheep tears had high concentrations of lysozyme, whereas cattle tears had low concentrations. Results of the microtiter plate colorometric assay suggested that llama tears had high concentrations of lysozyme, whereas concentrations in sheep and cattle tears were lower.
Lysozyme concentrations in tears may vary among species and this variability may contribute to differing susceptibilities to ocular diseases such as infectious keratoconjunctivitis.
确定美洲驼、绵羊和牛的眼泪中是否含有溶菌酶，并比较这些物种眼泪中溶菌酶的浓度。
40只美洲驼、5只绵羊和36头牛。
进行了电泳、溶菌酶的蛋白质印迹免疫测定、通过其溶解溶壁微球菌悬液的能力来检测眼泪溶菌酶的分光光度测定法以及微量滴定板比色测定法。
通过电泳和蛋白质印迹免疫测定在美洲驼和绵羊眼泪中检测到一条13.6-kd的蛋白带，但在牛眼泪中未检测到。分光光度测定结果表明美洲驼和绵羊眼泪中溶菌酶浓度高，而牛眼泪中浓度低。微量滴定板比色测定结果表明美洲驼眼泪中溶菌酶浓度高，而绵羊和牛眼泪中的浓度较低。
不同物种眼泪中的溶菌酶浓度可能不同，这种差异可能导致对诸如感染性角膜结膜炎等眼部疾病的易感性不同。
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