SK4在小鼠膀胱平滑肌细胞中编码中等电导的钙激活钾通道。
SK4 encodes intermediate conductance Ca2+-activated K+ channels in mouse urinary bladder smooth muscle cells.
作者信息
Ohya S, Kimura S, Kitsukawa M, Muraki K, Watanabe M, Imaizumi Y
机构信息
Department of Molecular and Cellular Pharmacology, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
出版信息
Jpn J Pharmacol. 2000 Sep;84(1):97-100. doi: 10.1254/jjp.84.97.
The single channel current of intermediate conductance Ca2 +-activated K+ channel (IK channel) was measured in mouse urinary bladder myocytes (MBM), and the molecular basis of the channel was suggested to be the SK4 subtype by RT-PCR. Among Ca2+-activated K+ channel subtypes (SK2, SK3, SK4 and BK), the mRNAs of SK4 and BK were predominantly expressed in MBM. IK channel currents recorded from MBM showed: 38.7 pS slope conductance under symmetrical 140 mM K+ conditions, Ca2+-dependent activation, and blockade by charybdotoxin and econazole. These results strongly suggest that SK4 encodes IK channels in MBM.
在小鼠膀胱肌细胞（MBM）中测量了中电导Ca2 + 激活的K + 通道（IK通道）的单通道电流，通过逆转录聚合酶链反应（RT-PCR）表明该通道的分子基础是SK4亚型。在Ca2 + 激活的K + 通道亚型（SK2、SK3、SK4和BK）中，SK4和BK的信使核糖核酸（mRNA）在MBM中主要表达。从MBM记录的IK通道电流显示：在对称的140 mM K + 条件下，斜率电导为38.7 pS，Ca2 + 依赖性激活，并被蝎毒素和益康唑阻断。这些结果强烈表明SK4编码MBM中的IK通道。
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