一种用于在体内测定蛋白质跨膜区段相互作用的基因融合方法。 - Suppr

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A gene fusion method for assaying interactions of protein transmembrane segments in vivo.

作者信息

Leeds J A, Beckwith J

机构信息

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.

出版信息

Methods Enzymol. 2000;327:165-75. doi: 10.1016/s0076-6879(00)27274-1.

DOI:10.1016/s0076-6879(00)27274-1

PMID:11044981

Abstract

摘要

相似文献

A gene fusion method for assaying interactions of protein transmembrane segments in vivo.

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引用本文的文献

The transmembrane domain of the DnaJ-like protein DjlA is a dimerisation domain.

Mol Genet Genomics. 2003 Mar;268(6):761-70. doi: 10.1007/s00438-002-0793-z. Epub 2003 Jan 31.

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A gene fusion method for assaying interactions of protein transmembrane segments in vivo.

作者信息

Leeds J A, Beckwith J

机构信息

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.

出版信息

Methods Enzymol. 2000;327:165-75. doi: 10.1016/s0076-6879(00)27274-1.

DOI:10.1016/s0076-6879(00)27274-1

PMID:11044981

Abstract

摘要

相似文献

A gene fusion method for assaying interactions of protein transmembrane segments in vivo.

Methods Enzymol. 2000;327:165-75. doi: 10.1016/s0076-6879(00)27274-1.

Tagging exported proteins using Escherichia coli alkaline phosphatase gene fusions.

Methods Enzymol. 2000;326:35-47. doi: 10.1016/s0076-6879(00)26045-x.

Lambda repressor N-terminal DNA-binding domain as an assay for protein transmembrane segment interactions in vivo.

J Mol Biol. 1998 Jul 31;280(5):799-810. doi: 10.1006/jmbi.1998.1893.

Analysis of Escherichia coli TonB membrane topology by use of PhoA fusions.

J Bacteriol. 1991 Sep;173(17):5554-7. doi: 10.1128/jb.173.17.5554-5557.1991.

Membrane topology of the N-terminus of the Escherichia coli FtsK division protein.

FEBS Lett. 2000 Jul 28;478(1-2):13-8. doi: 10.1016/s0014-5793(00)01820-2.

Export of FepA::PhoA fusion proteins to the outer membrane of Escherichia coli K-12.

J Bacteriol. 1989 Nov;171(11):5894-900. doi: 10.1128/jb.171.11.5894-5900.1989.

Topological study of Vibrio alginolyticus NhaB Na+/H+ antiporter using gene fusions in Escherichia coli cells.

Biochim Biophys Acta. 1998 Mar 6;1370(1):77-86. doi: 10.1016/s0005-2736(97)00245-9.

Alkaline phosphatase fusion proteins for molecular characterization and cloning of receptors and their ligands.

Methods Enzymol. 2000;327:198-210. doi: 10.1016/s0076-6879(00)27277-7.

Membrane topology of helices VII and XI in the lactose permease of Escherichia coli studied by lacY-phoA fusion analysis and site-directed spectroscopy.

Biochemistry. 1995 Nov 14;34(45):14909-17. doi: 10.1021/bi00045a036.

Genetic selection for and molecular dynamic modeling of a protein transmembrane domain multimerization motif from a random Escherichia coli genomic library.

J Mol Biol. 2001 Oct 12;313(1):181-95. doi: 10.1006/jmbi.2001.5007.

引用本文的文献

The transmembrane domain of the DnaJ-like protein DjlA is a dimerisation domain.

Mol Genet Genomics. 2003 Mar;268(6):761-70. doi: 10.1007/s00438-002-0793-z. Epub 2003 Jan 31.