Seraphim K R, de Siqueira M E
Escola de Farmácia e Odontologia de Alfenas, MG, Brasil.
Arch Latinoam Nutr. 2000 Mar;50(1):87-90.
The evaluation of nitrate and nitrite in homemade and industrialized cheeses commercialized in the southern region of Minas Gerais State, was the aim of this work. In Brazil, the use of these additives is permitted at maximum levels of 50 mg/kg. The basis of the previously validated method is the quantitative reduction of nitrate to nitrite through cadmium column and spectrophotometric determination after nitrite diazotation with sulphanilic acid/alpha-naphtol reagent. From all samples analyzed, 38 (88.37%) showed neither a nitrate nor nitrite detectable content; 5 samples (11.63%) presented nitrate, 4 of them being above the tolerated level and only one (2.33%) showed detectable nitrite, however below the permissible limit. The majority of samples did not show nitrate or nitrite at detectable levels. However, though probably safe from a toxicological point of view, the results can implicate an increased risk of bacteriological contamination.
本研究旨在评估米纳斯吉拉斯州南部地区商业化销售的自制奶酪和工业化生产奶酪中的硝酸盐和亚硝酸盐含量。在巴西,这些添加剂的最大使用量允许为50毫克/千克。先前经过验证的方法的基础是通过镉柱将硝酸盐定量还原为亚硝酸盐,并在用磺胺酸/α-萘酚试剂进行亚硝酸盐重氮化后进行分光光度测定。在所有分析的样品中,38个(88.37%)既未检测到硝酸盐也未检测到亚硝酸盐含量;5个样品(11.63%)含有硝酸盐,其中4个高于耐受水平,只有1个(2.33%)检测到亚硝酸盐,但低于允许限度。大多数样品未检测到可检测水平的硝酸盐或亚硝酸盐。然而,尽管从毒理学角度来看可能是安全的,但结果可能意味着细菌污染风险增加。