Tsujiuchi T, Sasaki Y, Tsutsumi M, Konishi Y
Department of Oncological Pathology, Cancer Center, Nara Medical University, Kashihara, Nara 634-8521, Japan.
Jpn J Cancer Res. 2000 Oct;91(10):955-9. doi: 10.1111/j.1349-7006.2000.tb00870.x.
The expression of interleukins (ILs) in lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine (BHP) in rats was investigated using a multiprobe RNase protection assay (RPA) followed by densitometric quantification. Male Wistar rats, 6 weeks old, were given 2000 ppm BHP in their drinking water for 12 weeks and maintained without further treatment until they were killed at week 25. Total RNAs were extracted from 14 individual adenocarcinomas and 2 specimens of normal lung tissue of untreated rats. In adenocarcinomas, elevated expression of IL-1alpha (6 / 14), IL-1beta (14 / 14), IL-3 (7 / 14), IL-4 (11 / 14), IL-5 (9 / 14), IL-6 (11 / 14) and IL-10 (8 / 14) was observed, compared with normal lung tissues. In contrast, no expression of IL-2 was detected in any case. The results suggest that preferential expression of these ILs and their complex networks may contribute to the development and progression of lung adenocarcinomas induced by BHP in rats.
使用多探针核糖核酸酶保护分析（RPA）并进行光密度定量分析，研究了N-亚硝基双（2-羟丙基）胺（BHP）诱导的大鼠肺腺癌中白细胞介素（ILs）的表达情况。6周龄雄性Wistar大鼠饮用含2000 ppm BHP的水12周，之后不再接受进一步处理，直至在第25周处死。从未经处理大鼠的14个单个腺癌组织和2个正常肺组织标本中提取总RNA。与正常肺组织相比，在腺癌中观察到IL-1α（6/14）、IL-1β（14/14）、IL-3（7/14）、IL-4（11/14）、IL-5（9/14）、IL-6（11/14）和IL-10（8/14）的表达升高。相反，在任何情况下均未检测到IL-2的表达。结果表明，这些白细胞介素的优先表达及其复杂网络可能有助于BHP诱导的大鼠肺腺癌的发生和发展。
