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Purification, crystallization and preliminary diffraction study of beta-galactosidase from Penicillium sp.

作者信息

Neustroev K N, de Sousa E A, Golubev A M, Eneyskaya E V, Kulminskaya A A, Polikarpov I

机构信息

Petersburg Nuclear Physics Institute, Gatchina, St Petersburg, 188350, Russia.

出版信息

Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1508-9. doi: 10.1107/s0907444900011756.

Abstract

Crystals of an extracellular beta-galactosidase from Penicillium sp. (MW = 120 +/- 5 kDa) have been obtained from a sodium phosphate buffer using PEG as precipitant. The crystals belong to the tetragonal space group P4(1)or P4(3), with unit-cell parameters a = b = 110.82, c = 161.28 A, and diffract to 1.85 A resolution at a synchrotron source.

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