Tinguely M, Bründler M A, Gogos S, Kerl K, Borisch B
Department of Clinical Pathology, University Hospital of Geneva, Switzerland.
Arch Immunol Ther Exp (Warsz). 2000;48(4):317-22.
Recent studies have shown marked geographic variation associated with Epstein-Barr virus (EBV) in pediatric Burkitt's lymphomas and Hodgkin's disease. In the present study we investigated 30 cases of pediatric extranodal high grade non-Hodgkin's lymphomas (NHL) from Turkey with an abdominal localisation. To classify them histologically and to determine the role of EBV in these lymphomas, immunohistochemistry (IHC), in situ hybridisation (ISH) and polymerase chain reaction (PCR) were used. Our series contained two histologic types: the Burkitt's or Burkitt's-like lymphomas (BL/BLL) and high grade NHL. They all were of the B cell type. The immunoglobulin heavy chain gene rearrangement revealed monoclonality in 87% of the BL/BLL cases, in contrast to the NHL cases, showing monoclonality in only 43% of the cases. EBV was found in tumor cells in a high frequency, independent of the histological subtype. EBV strains A and B were detected in 9 cases, with a preponderance of the B subtype (4/9 BL/BLL; 4/9 NHL). Our data suggest that high grade NHLs with abdominal localisation of Turkish children show the pattern of immunodeficient lymphomas to some extent.
最近的研究表明，小儿伯基特淋巴瘤和霍奇金病中与爱泼斯坦-巴尔病毒（EBV）相关的地理差异显著。在本研究中，我们调查了30例来自土耳其的腹部定位的小儿结外高级别非霍奇金淋巴瘤（NHL）。为了对它们进行组织学分类并确定EBV在这些淋巴瘤中的作用，我们使用了免疫组织化学（IHC）、原位杂交（ISH）和聚合酶链反应（PCR）。我们的系列包含两种组织学类型：伯基特淋巴瘤或伯基特样淋巴瘤（BL/BLL）和高级别NHL。它们均为B细胞型。免疫球蛋白重链基因重排显示，87%的BL/BLL病例呈单克隆性，相比之下，NHL病例仅43%呈单克隆性。在肿瘤细胞中高频发现EBV，与组织学亚型无关。在9例中检测到EBV A和B株，其中B亚型占优势（4/9 BL/BLL；4/9 NHL）。我们的数据表明，土耳其儿童腹部定位的高级别NHL在一定程度上表现出免疫缺陷淋巴瘤的模式。
