Chang C C, Eshoa C, Kampalath B, Shidham V B, Perkins S
Dept of Pathology, Medical College of Wisconsin, Milwaukee 53006, USA.
Am J Clin Pathol. 2000 Nov;114(5):807-11. doi: 10.1309/WWW7-DG6X-HC16-D7J2.
The present study was designed to evaluate the lineage differentiation (particularly monocytic differentiation) of immature myeloid cells in granulocytic sarcoma (GS) by immunohistochemistry and correlate the results with lineage differentiation of blasts in the bone marrow and to determine the degree of maturation of the infiltrating myeloid cells in GS by immunohistochemistry using CD34 and HLA-DR. Immunohistochemical stains were performed on paraffin-embedded tissue from 17 GS lesions with lineage-associated markers: myeloperoxidase, CD68 (KP1), CD68 (PG-Ml), glycophorin A, factor VIII, and CD56; and with markers for blasts and immature myeloid cells: CD34 and HLA-DR. Our results show that positive staining with PG-M1, but not KP1, suggests monocytic differentiation of myeloid cells in GS and correlates with the monocytic differentiation of blasts in the bone marrow. Expression of CD56 is frequent in GS, especially when the marrow blasts have monocytic differentiation, and should not be interpreted as a primary natural-killer cell process. The immature myeloid cells in GS are frequently HLA-DR positive. However, CD34 positivity of the immature myeloid cells is relatively uncommon, except in cases with underlying myelodysplastic syndrome or chronic myelogenous leukemia.
本研究旨在通过免疫组织化学评估粒细胞肉瘤（GS）中未成熟髓样细胞的谱系分化（尤其是单核细胞分化），并将结果与骨髓中原始细胞的谱系分化相关联，同时通过使用CD34和HLA-DR的免疫组织化学确定GS中浸润性髓样细胞的成熟程度。对来自17个GS病变的石蜡包埋组织进行免疫组织化学染色，使用与谱系相关的标志物：髓过氧化物酶、CD68（KP1）、CD68（PG-M1）、血型糖蛋白A、因子VIII和CD56；以及用于原始细胞和未成熟髓样细胞的标志物：CD34和HLA-DR。我们的结果表明，PG-M1而非KP1的阳性染色提示GS中髓样细胞的单核细胞分化，并与骨髓中原始细胞的单核细胞分化相关。CD56在GS中表达频繁，尤其是当骨髓原始细胞具有单核细胞分化时，不应将其解释为原发性自然杀伤细胞过程。GS中的未成熟髓样细胞经常HLA-DR阳性。然而，未成熟髓样细胞的CD34阳性相对少见，除非存在潜在的骨髓增生异常综合征或慢性粒细胞白血病。
