Windels F, Bruet N, Poupard A, Urbain N, Chouvet G, Feuerstein C, Savasta M
Equipe Neurochimie et Neuroplasticité Fonctionnelles, INSERM U.318- Neurosciences Précliniques, Université Joseph Fourier, Pavillon de Neurologie, CHU de Grenoble, 38043 Grenoble Cedex 09, France.
Eur J Neurosci. 2000 Nov;12(11):4141-6. doi: 10.1046/j.1460-9568.2000.00296.x.
High frequency stimulation (130 Hz) of the subthalamic nucleus has dramatic beneficial motor effects in severe parkinsonian patients. However, the mechanisms underlying these clinical results remain obscure. The objective of the present work was to study the neurochemical changes induced in rats by high frequency stimulation of the subthalamic nucleus by using intracerebral microdialysis within its target structures. Our results show that high frequency stimulation of the subthalamic nucleus induces a significant increase of extracellular glutamate levels in the ipsilateral globus pallidus and substantia nigra while GABA was augmented only in the substantia nigra. These data suggest that functional effects induced by high frequency stimulation of the subthalamic nucleus might imply distal mechanisms involving the synaptic relationships with the subthalamic efferences. They question the current view that the direct inhibition of the subthalamic neurons is induced by high frequency stimulation.
高频刺激（130赫兹）丘脑底核对重度帕金森病患者具有显著的有益运动效果。然而，这些临床结果背后的机制仍不清楚。本研究的目的是通过在大鼠丘脑底核的目标结构内使用脑内微透析技术，研究高频刺激丘脑底核对大鼠诱导的神经化学变化。我们的结果表明，高频刺激丘脑底核会导致同侧苍白球和黑质细胞外谷氨酸水平显著升高，而γ-氨基丁酸（GABA）仅在黑质中增加。这些数据表明，高频刺激丘脑底核所诱导的功能效应可能涉及与丘脑底核传出纤维突触关系的远端机制。它们对当前认为高频刺激可直接抑制丘脑底核神经元的观点提出了质疑。
