人类免疫缺陷病毒对人类CD4（-）细胞的非Env依赖性感染。
Human immunodeficiency virus Env-independent infection of human CD4(-) cells.
作者信息
Pang S, Yu D, An D S, Baldwin G C, Xie Y, Poon B, Chow Y H, Park N H, Chen I S
机构信息
Division of Oral Biology and Medicine, UCLA School of Dentistry, Los Angeles, California 90095, USA.
出版信息
J Virol. 2000 Dec;74(23):10994-1000. doi: 10.1128/jvi.74.23.10994-11000.2000.
Abstract
CD4(-) epithelial cells covering mucosal surfaces serve as the primary barrier to prevent human immunodeficiency virus type 1 (HIV-1) infection. We used HIV-1 vectors carrying the enhanced green fluorescent protein gene as a reporter gene to demonstrate that HIV-1 can infect some CD4(-) human epithelial cell lines with low but significant efficiencies. Importantly, HIV-1 infection of these cell lines is independent of HIV-1 envelope proteins. The Env-independent infection of CD4(-) cells by HIV-1 suggests an alternative pathway for HIV-1 transmission. Even on virions bearing Env, a neutralizing antibody directed against gp120 is incapable of neutralizing the infection of these cells, thus raising potential implications for HIV-1 vaccine development.
摘要
覆盖黏膜表面的CD4（-）上皮细胞是预防1型人类免疫缺陷病毒（HIV-1）感染的主要屏障。我们使用携带增强型绿色荧光蛋白基因作为报告基因的HIV-1载体，以证明HIV-1能够以低但显著的效率感染一些CD4（-）人类上皮细胞系。重要的是，这些细胞系的HIV-1感染不依赖于HIV-1包膜蛋白。HIV-1对CD4（-）细胞的非Env依赖性感染提示了HIV-1传播的另一条途径。即使在带有Env的病毒粒子上，针对gp120的中和抗体也无法中和这些细胞的感染，从而对HIV-1疫苗开发产生潜在影响。
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