Bacigalupo M A, Ius A, Longhi R, Meroni G
Istituto di Biocatalisi e Riconoscimento Molecolare, CNR, Milan, Italy.
Analyst. 2000 Oct;125(10):1847-50. doi: 10.1039/b003481i.
In the control of tomato transgenic cultivars it is important to determine the glycoalkaloid content, which requires the screening of many samples. We have developed a simple method for the extraction and determination of glycoalkaloids in the leaf and fruit extracts of tomatoes using a europium chelator entrapped in phosphatidylcholine-cholesterol containing liposomes. The concentration values were quantified from liposome lysis effected by glycoalkaloid action. The fluorescent signal is linear between 1 and 10 micrograms of tomatine, which makes the method useful for the analysis of tomato samples.
在番茄转基因品种的控制中，确定糖苷生物碱含量很重要，这需要对许多样品进行筛选。我们开发了一种简单的方法，使用包裹在含磷脂酰胆碱 - 胆固醇脂质体中的铕螯合剂来提取和测定番茄叶片和果实提取物中的糖苷生物碱。通过糖苷生物碱作用引起的脂质体裂解来定量浓度值。荧光信号在1至10微克番茄碱之间呈线性，这使得该方法可用于番茄样品的分析。
