Rostral ventrolateral medulla suppresses reflex bradycardia by the release of gamma-aminobutyric acid in nucleus tractus solitarii of the rat.

We investigated the role of gamma-aminobutyric acid (GABA) in the nucleus tractus solitarii (NTS), the principal recipient of baroreceptor afferent fibers in the medulla oblongata, in the suppression of cardiac baroreceptor reflex (BRR) response by the rostral ventrolateral medulla (RVLM). Direct microinfusion via reverse microdialysis of L-glutamate (50 microM) into the RVLM promoted an inhibition of the BRR response, alongside an increase in the concentration of GABA in the dialysate collected from the ipsilateral NTS. Such an increase in GABA concentration in the NTS to RVLM activation was site-specific, as microinfusion of L-glutamate into areas outside the confines of RVLM resulted in no discernible change in GABA concentration in the dialysate of the NTS and minimal effect on the cardiac BRR response. The RVLM-induced BRR suppression of cardiac BRR response to microinjection into the bilateral RVLM of L-glutamate (1 nmol) was antagonized by administration into the bilateral NTS of the GABA(A) receptor antagonist, bicuculline methiodide (1 or 5 pmol), or the GABA(B) receptor antagonist, 2-hydroxy-saclofen (100 or 500 pmol). These results suggest that GABA released in the NTS may participate in cardiac BRR suppression induced by glutamatergic activation of the RVLM, via an action on both GABA(A) and GABA(B) receptor subtypes.