大肠杆菌ccm突变体的种间互补：来自日本慢生根瘤菌的CcmE（CycJ）在细胞色素c成熟过程中作为血红素伴侣发挥作用。
Interspecies complementation of Escherichia coli ccm mutants: CcmE (CycJ) from Bradyrhizobium japonicum acts as a heme chaperone during cytochrome c maturation.
作者信息
Schulz H, Thöny-Meyer L
机构信息
Institut für Mikrobiologie, Eidgenössische Technische Hochschule, CH 8092 Zürich, Switzerland.
出版信息
J Bacteriol. 2000 Dec;182(23):6831-3. doi: 10.1128/JB.182.23.6831-6833.2000.
Abstract
Biogenesis of c-type cytochromes in alpha- and gamma-proteobacteria requires the function of a set of orthologous genes (ccm genes) that encode specific maturation factors. The Escherichia coli CcmE protein is a periplasmic heme chaperone. The membrane protein CcmC is required for loading CcmE with heme. By expressing CcmE (CycJ) from Bradyrhizobium japonicum in E. coli we demonstrated that heme is bound covalently to this protein at a strictly conserved histidine residue. The B. japonicum homologue can transfer heme to apocytochrome c in E. coli, suggesting that it functions as a heme chaperone. CcmC (CycZ) from B. japonicum expressed in E. coli was capable of inserting heme into CcmE.
摘要
α-和γ-变形菌纲中c型细胞色素的生物合成需要一组编码特定成熟因子的直系同源基因（ccm基因）发挥作用。大肠杆菌的CcmE蛋白是一种周质血红素伴侣蛋白。膜蛋白CcmC是CcmE加载血红素所必需的。通过在大肠杆菌中表达来自日本慢生根瘤菌的CcmE（CycJ），我们证明血红素在一个严格保守的组氨酸残基处与该蛋白共价结合。日本慢生根瘤菌的同源物可以将血红素转移至大肠杆菌中的脱辅基细胞色素c，这表明它作为一种血红素伴侣蛋白发挥作用。在大肠杆菌中表达的来自日本慢生根瘤菌的CcmC（CycZ）能够将血红素插入CcmE。
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