通过细针穿刺细胞学材料的DNA流式细胞术对精子发生进行定量分析。

Quantitation of spermatogenesis by DNA flow cytometry from fine-needle aspiration cytology material.

作者信息

Dey P, Mondal A K, Singh S K, Vohra H

机构信息

Department of Cytology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.

出版信息

Diagn Cytopathol. 2000 Dec;23(6):386-7. doi: 10.1002/1097-0339(200012)23:6<386::aid-dc4>3.0.co;2-s.

DOI:10.1002/1097-0339(200012)23:6<386::aid-dc4>3.0.co;2-s

PMID:11074642

Abstract

DNA flow cytometry (FCM) was performed from fine-needle aspiration cytology (FNAC) of testis in 15 cases of male infertility to quantitate spermatogenesis. The results were correlated with FNAC findings. DNA FCM showed a ploidy relationship of haploid (1N) > diploid (2N) > tetraploid (4N) in cases of normal spermatogenesis. A ploidy relation of 2N > 1N > 4N was observed in cases of hypospermatogenesis or maturation arrest. In Sertoli cell-only cases, there were only 2N populations of cells. With the help of DNA FCM, a rapid and objective assessment of spermatogenesis is possible from FNAC of the testis.

摘要

对15例男性不育患者的睾丸细针穿刺抽吸细胞学检查（FNAC）样本进行DNA流式细胞术（FCM），以对精子发生进行定量分析。结果与FNAC检查结果相关。DNA FCM显示，在精子发生正常的病例中，单倍体（1N）>二倍体（2N）>四倍体（4N）的倍性关系。在精子发生低下或成熟停滞的病例中，观察到2N>1N>4N的倍性关系。在仅有支持细胞的病例中，仅存在2N的细胞群。借助DNA FCM，可通过睾丸FNAC对精子发生进行快速、客观的评估。