内皮素-2/血管活性肠收缩基因：在大鼠嗜铬细胞瘤PC12细胞中的表达及启动子活性

The endothelin-2/vasoactive intestinal contractor gene: expression and promoter activity in PC12 rat pheochromocytoma cells.

作者信息

Saida K, Uchide T, Usui A, Gao X D, Tomizuka N, Oka S, Masuda H

机构信息

Biosignaling Department, National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Tsukuba, Ibaraki, Japan.

出版信息

J Cardiovasc Pharmacol. 2000 Nov;36(5 Suppl 1):S9-11. doi: 10.1097/00005344-200036051-00005.

DOI:10.1097/00005344-200036051-00005

PMID:11078321

Abstract

In order to understand the physiological roles of vasoactive intestinal contractor (VIC)/endothelin-2 (ET-2), we examined the expression of this peptide by specific reverse transcriptase polymerase chain reaction (RT-PCR) analysis and found that PC12 rat pheochromocytoma cells express the VIC gene. The 5'-flanking 1.0 kilo base pair (kb) region of the mouse VIC gene is sufficient to express a secreted alkaline phosphatase (SEAP) reporter gene in transiently transfected PC12 cells. The 1.0 kb promoter region may contain cis-acting elements that determine the rate of the VIC gene transcription in PC12 cells.

摘要

为了了解血管活性肠收缩肽（VIC）/内皮素-2（ET-2）的生理作用，我们通过特异性逆转录聚合酶链反应（RT-PCR）分析检测了该肽的表达，发现PC12大鼠嗜铬细胞瘤细胞表达VIC基因。小鼠VIC基因5'侧翼1.0千碱基对（kb）区域足以在瞬时转染的PC12细胞中表达分泌性碱性磷酸酶（SEAP）报告基因。1.0 kb的启动子区域可能包含决定PC12细胞中VIC基因转录速率的顺式作用元件。