McDermott M T, Haugen B R, Gordon D F, Wood W M, Brown N S, Bauer C A, Garrity M J, Kleinschmidt-DeMasters B K, Lillehei K O, Samuels M H, Bright T M, Ridgway E C
Division of Endocrinology, University of Colorado Health Sciences Center, Denver 80262, USA.
Pituitary. 1999 Nov;2(3):217-24. doi: 10.1023/a:1009957411973.
Pit-1 is a transcription factor that appears early in embryonic pituitary gland formation and is necessary for the development of somatotropes, lactotropes and thyrotropes. Steroidogenic factor-1 (SF-1) is another early appearing transcription factor that is involved in the development of gonadotropes. In this study we have compared RT-PCR analysis of hormone mRNA with traditional IHC for classification of 27 pituitary tumors and have evaluated the correlation of Pit-1 and SF-1 mRNA with hormone mRNA. RT-PCR detected concordant hormone mRNA in 100% of GH IHC positive, 100% of PRL IHC positive, 33% of TSH IHC positive, and 93% of gonadotropin IHC positive tumors. IHC, however, was concordant in only 71% of GH mRNA positive, 78% of PRL mRNA positive, 17% of TSH beta mRNA positive, and 76% of FSH beta mRNA positive tumors. Pit-1 mRNA was positive in 87% of tumors in which mRNA for GH, PRL or TSH beta was detected and in only 17% of GH, PRL and TSH beta mRNA negative tumors. SF-1 mRNA was positive in 94% of tumors in which mRNA for FSH beta was present and in no FSH beta mRNA negative tumors. We conclude that RT-PCR analysis of hormone mRNA may be more sensitive than traditional hormone IHC for classification of pituitary tumors. Furthermore, tumor Pit-1 mRNA positively correlates with GH, PRL and TSH beta mRNA while tumor SF-1 mRNA correlates well with FSH beta mRNA. Combined analysis of hormone and transcription factor mRNA in pituitary tumor tissue may therefore be a more meaningful approach to pituitary tumor characterization.
垂体特异性转录因子1（Pit-1）是一种在胚胎垂体形成早期出现的转录因子，对生长激素细胞、催乳素细胞和促甲状腺激素细胞的发育至关重要。类固醇生成因子1（SF-1）是另一种早期出现的转录因子，参与促性腺激素细胞的发育。在本研究中，我们比较了激素mRNA的逆转录聚合酶链反应（RT-PCR）分析与传统免疫组化（IHC）对27例垂体肿瘤的分类，并评估了Pit-1和SF-1 mRNA与激素mRNA的相关性。RT-PCR在100%的生长激素免疫组化阳性、100%的催乳素免疫组化阳性、33%的促甲状腺激素免疫组化阳性和93%的促性腺激素免疫组化阳性肿瘤中检测到一致的激素mRNA。然而，免疫组化在仅71%的生长激素mRNA阳性、78%的催乳素mRNA阳性、17%的促甲状腺激素β mRNA阳性和76%的促卵泡激素β mRNA阳性肿瘤中结果一致。在检测到生长激素、催乳素或促甲状腺激素β mRNA的肿瘤中，87%的肿瘤Pit-1 mRNA呈阳性，而在生长激素、催乳素和促甲状腺激素β mRNA阴性的肿瘤中，只有17%呈阳性。在存在促卵泡激素β mRNA的肿瘤中，94%的肿瘤SF-1 mRNA呈阳性，而在促卵泡激素β mRNA阴性的肿瘤中均未检测到阳性。我们得出结论，对于垂体肿瘤的分类，激素mRNA的RT-PCR分析可能比传统的激素免疫组化更敏感。此外，肿瘤Pit-1 mRNA与生长激素、催乳素和促甲状腺激素β mRNA呈正相关，而肿瘤SF-1 mRNA与促卵泡激素β mRNA相关性良好。因此，垂体肿瘤组织中激素和转录因子mRNA的联合分析可能是一种更有意义的垂体肿瘤特征分析方法。
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