有丝分裂器处的CPEB、maskin和细胞周期蛋白B1信使核糖核酸：对细胞分裂的局部翻译控制的影响

CPEB, maskin, and cyclin B1 mRNA at the mitotic apparatus: implications for local translational control of cell division.

作者信息

Groisman I, Huang Y S, Mendez R, Cao Q, Theurkauf W, Richter J D

机构信息

Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655, USA.

出版信息

Cell. 2000 Oct 27;103(3):435-47. doi: 10.1016/s0092-8674(00)00135-5.

DOI:10.1016/s0092-8674(00)00135-5

PMID:11081630

Abstract

In Xenopus development, the expression of several maternal mRNAs is regulated by cytoplasmic polyadenylation. CPEB and maskin, two factors that control polyadenylation-induced translation are present on the mitotic apparatus of animal pole blastomeres in embryos. Cyclin B1 protein and mRNA, whose translation is regulated by polyadenylation, are colocalized with CPEB and maskin. CPEB interacts with microtubules and is involved in the localization of cyclin B1 mRNA to the mitotic apparatus. Agents that disrupt polyadenylation-induced translation inhibit cell division and promote spindle and centrosome defects in injected embryos. Two of these agents inhibit the synthesis of cyclin B1 protein and one, which has little effect on this process, disrupts the localization of cyclin B1 mRNA and protein. These data suggest that CPEB-regulated mRNA translation is important for the integrity of the mitotic apparatus and for cell division.

摘要

在非洲爪蟾的发育过程中，几种母源mRNA的表达受细胞质多聚腺苷酸化调控。CPEB和maskin这两种控制多聚腺苷酸化诱导翻译的因子存在于胚胎动物极卵裂球的有丝分裂器上。细胞周期蛋白B1的蛋白质和mRNA，其翻译受多聚腺苷酸化调控，与CPEB和maskin共定位。CPEB与微管相互作用，并参与细胞周期蛋白B1 mRNA定位于有丝分裂器的过程。破坏多聚腺苷酸化诱导翻译的试剂会抑制注射胚胎中的细胞分裂，并促进纺锤体和中心体缺陷。其中两种试剂抑制细胞周期蛋白B1蛋白质的合成，而另一种对这一过程影响很小，它会破坏细胞周期蛋白B1 mRNA和蛋白质的定位。这些数据表明，CPEB调控的mRNA翻译对于有丝分裂器的完整性和细胞分裂很重要。