Sang Y, Liang G H
Department of Agronomy, Kansas State University, Manhattan 66506-5501, USA.
Genome. 2000 Oct;43(5):918-22.
The physical locations of the 18S-5.8S-26S rDNA sequences were examined in three sorghum species by fluorescence in situ hybridization (FISH) using biotin-labeled heterologous 18S-5.8S-26S rDNA probe (pTa71). Each 18S-5.8S-26S rDNA locus occurred at two sites on the chromosomes in Sorghum bicolor (2n = 20) and S. versicolor (2n = 10), but at four sites on the chromosomes of S. halepense (2n = 40) and the tetraploid S. versicolor (2n = 20). Positions of the rDNA loci varied from the interstitial to terminal position among the four accessions of the three sorghum species. The rDNA data are useful for investigation of chromosome evolution and phylogeny. This study excluded S. versicolor as the possible progenitor of S. bicolor.
利用生物素标记的异源18S - 5.8S - 26S rDNA探针（pTa71），通过荧光原位杂交（FISH）技术，在三种高粱属物种中检测了18S - 5.8S - 26S rDNA序列的物理位置。在双色高粱（2n = 20）和异色高粱（2n = 10）中，每个18S - 5.8S - 26S rDNA位点出现在染色体的两个位置上，但在黑高粱（2n = 40）和四倍体异色高粱（2n = 20）的染色体上则出现在四个位置上。在这三种高粱属物种的四个材料中，rDNA位点的位置从染色体中间到末端各不相同。rDNA数据对于研究染色体进化和系统发育很有用。本研究排除了异色高粱作为双色高粱可能祖先的可能性。
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