Aburjai T, Amro B I, Aiedeh K, Abuirjeie M, al-Khalil S
Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, Amman, Jordan.
Pharmazie. 2000 Oct;55(10):751-4.
Two accurate, precise and sensitive thin layer chromatographic (TLC) and second derivative UV-spectrophotometric procedures are described for the simultaneous determination of ascorbic acid and dipyrone in pure form and in pharmaceutical dosage forms. The TLC method involved direct application of methanolic solutions of tested samples on silica gel TLC plates using water:methanol (95:5 v/v) as developing system. The developed plates were then directly scanned at 260 nm using a TLC scanner. The second method depends on second derivative UV-spectrophotometry with zero crossing technique of measurement. Second derivative amplitudes at 280 and 272 nm were selected for the determination of ascorbic acid and dipyrone, respectively. Both methods show good linearity, precision and reproducibility. They are simple and do not require manipulation prior to analysis. The proposed methods have been successfully applied to the determination of the drugs in various pharmaceutical dosage forms such as tablets and ampoules.
本文描述了两种准确、精密且灵敏的薄层色谱（TLC）和二阶导数紫外分光光度法，用于同时测定纯品及药物剂型中的抗坏血酸和安乃近。TLC法是将待测样品的甲醇溶液直接点样于硅胶TLC板上，以水：甲醇（95:5 v/v）作为展开系统。然后使用TLC扫描仪在260 nm处直接扫描展开后的薄板。第二种方法基于采用零交叉测量技术的二阶导数紫外分光光度法。分别选择280 nm和272 nm处的二阶导数振幅来测定抗坏血酸和安乃近。两种方法均显示出良好的线性、精密度和重现性。它们操作简单，分析前无需预处理。所提出的方法已成功应用于各种药物剂型（如片剂和安瓿剂）中药物的测定。
