Cryan J R, Wiegmann B M, Deitz L L, Dietrich C H
Department of Entomology, North Carolina State University, Raleigh, North Carolina 27695-7613, USA.
Mol Phylogenet Evol. 2000 Nov;17(2):317-34. doi: 10.1006/mpev.2000.0832.
We present a molecular systematic investigation of relationships among family-group taxa of Membracidae, comprising nearly 3.5 kb of nucleotide sequence data from the nuclear genes elongation factor-1alpha (EF-1alpha: 958 bp) and 28S ribosomal DNA (28S rDNA: 2363 bp); data partitions are analyzed separately and in combination for 79 taxa. Analysis of the combined sequence data provided a better-resolved and more robust hypothesis of membracid phylogeny than did separate analyses of the individual genes. Results support the monophyly of the family Membracidae and indicate the presence of two major lineages (Centrotinae + Stegaspidinae + Centrodontinae and Darninae + Membracinae + Smiliinae). Within Membracidae, molecular data support the following assertions: (1) the previously unplaced genera Antillotolania and Deiroderes form a monophyletic group with Microcentrini; (2) Centrodontini and Nessorhinini are monophyletic clades that arise independently from within the Centrotinae; (3) Centrotinae is paraphyletic with respect to Centrodontinae; (4) the subfamily Membracinae is monophyletic and possibly allied with the darnine tribe Cymbomorphini; (5) the subfamily Darninae is paraphyletic; (6) the subfamily Smiliinae is paraphyletic, with molecular evidence indicating the exclusion of Micrutalini and perhaps Acutalini and Ceresini; and (7) Membracidae arose and diversified in the New World with multiple subsequent colonizations of the Old World. Our phylogenetic results suggest that morphology-based classifications of the Membracidae need to be reevaluated in light of emerging molecular evidence.
我们展示了一项关于角蝉科科级分类单元间亲缘关系的分子系统学研究，该研究包含来自核基因延伸因子-1α（EF-1α：958 bp）和28S核糖体DNA（28S rDNA：2363 bp）的近3.5 kb核苷酸序列数据；对79个分类单元分别及合并分析了数据分区。与对单个基因的单独分析相比，对合并序列数据的分析为角蝉科系统发育提供了一个解析度更高且更稳健的假说。结果支持角蝉科的单系性，并表明存在两个主要谱系（角蝉亚科+冠角蝉亚科+齿角蝉亚科以及达纳角蝉亚科+角蝉亚科+斯米利角蝉亚科）。在角蝉科内，分子数据支持以下论断：（1）先前未归位的属安的列托拉尼亚属和德罗德雷斯属与微角蝉族形成一个单系类群；（2）齿角蝉族和鼻角蝉族是分别独立起源于角蝉亚科内部的单系分支；（3）相对于齿角蝉亚科，角蝉亚科是并系的；（4）角蝉亚科是单系的，并且可能与达纳角蝉族的 Cymbomorphini 族相关联；（5）达纳角蝉亚科是并系的；（6）斯米利角蝉亚科是并系的，分子证据表明微突角蝉族以及可能的锐突角蝉族和谷角蝉族被排除在外；（7）角蝉科起源于新大陆并在那里分化，随后多次向旧大陆扩散。我们的系统发育结果表明，鉴于新出现的分子证据，基于形态学的角蝉科分类需要重新评估。
