Frutos Cabanillas P, Díez Peña E, Barrales-Rienda J M, Frutos G
Departamento de Química-Física de Polímeros. Instituto de Ciencia y Tecnología de Polímeros. Consejo Superior de Investigaciones Científicas (C.S.I.C.). Juan de la Cierva, 3. E-28006, Madrid, Spain.
Int J Pharm. 2000 Nov 19;209(1-2):15-26. doi: 10.1016/s0378-5173(00)00520-2.
Antibiotic-loaded polymeric bone cement is used in orthopedic surgery to deliver local high concentrations of antibiotics to the tissues. The precise mechanism by which antibiotic is released from the polymeric matrix is still not very well known. This research was conducted to investigate the in vitro drug release behavior of antibiotic from acrylic bone cement. A spectrophotometric method for the quantitative analysis of gentamicin sulfate using o-phtaldialdehyde as a derivatizing reagent was thoroughly validated, in order to assure a minimum quality level of the measures. The method proved to be quick, reliable, less expensive than methods such as polarization fluorescence immunoassay and others, and therefore more convenient for the routine analysis of the numerous samples generated during in vitro liberation assays. The release of gentamicin from commercial CMW1 acrylic bone cement samples was investigated following proposed in vitro release experiments.
载抗生素的聚合物骨水泥用于骨科手术，以便向组织输送高浓度的局部抗生素。抗生素从聚合物基质中释放的确切机制仍不太清楚。本研究旨在调查抗生素从丙烯酸骨水泥中的体外药物释放行为。使用邻苯二甲醛作为衍生试剂对硫酸庆大霉素进行定量分析的分光光度法得到了充分验证，以确保测量的最低质量水平。该方法被证明快速、可靠，比偏振荧光免疫分析等方法成本更低，因此更便于对体外释放试验中产生的大量样品进行常规分析。按照提议的体外释放实验，研究了庆大霉素从市售CMW1丙烯酸骨水泥样品中的释放情况。
