Georgas K, Bowles J, Yamada T, Koopman P, Little M H
Institute for Molecular Bioscience, incorporating the Centre for Molecular and Cellular Biology and the Special Research Centre for Functional and Applied Genomics, The University of Queensland, Brisbane, Australia.
Dev Dyn. 2000 Dec;219(4):582-7. doi: 10.1002/1097-0177(2000)9999:9999<::AID-DVDY1072>3.0.CO;2-I.
The Crim1 gene encodes a putative transmembrane protein with an IGF-binding protein motif and multiple chordin-like cysteine-rich repeats. In chordin, such repeats are responsible for its dorsalising activity and for binding to bone morphogenic proteins (BMPs). Crim1 displays a dynamic expression pattern in a variety of developing organs, including the CNS and the lens. We have undertaken a detailed expression pattern analysis of Crim1 in the developing mouse urogenital system. During metanephric development, Crim1 showed expression both in the ureteric tree, the early condensing mesenchyme and distal comma-shaped bodies. As the nephron elongates, Crim1 becomes expressed in the proximal end of the S-shaped bodies. Crim1 also displays a striking male-specific expression pattern in the fetal gonads, its expression strongest in the Sertoli cells of the developing testis.
Crim1基因编码一种假定的跨膜蛋白，该蛋白具有胰岛素样生长因子结合蛋白基序和多个类脊索蛋白富含半胱氨酸的重复序列。在脊索蛋白中，此类重复序列负责其背侧化活性以及与骨形态发生蛋白（BMPs）的结合。Crim1在包括中枢神经系统和晶状体在内的多种发育器官中呈现动态表达模式。我们对发育中的小鼠泌尿生殖系统进行了Crim1详细的表达模式分析。在肾后肾发育过程中，Crim1在输尿管树、早期凝聚的间充质和远端逗号状小体中均有表达。随着肾单位的延长，Crim1在S形小体的近端开始表达。Crim1在胎儿性腺中还呈现出显著的雄性特异性表达模式，其在发育中的睾丸支持细胞中表达最强。
