在CREBalphadelta基因敲除突变小鼠中，恐惧增强的惊吓反应受损，但前脉冲抑制惊吓反应未受影响。

Fear-potentiated startle, but not prepulse inhibition of startle, is impaired in CREBalphadelta-/- mutant mice.

作者信息

Falls W A, Kogan J H, Silva A J, Willott J F, Carlson S, Turner J G

机构信息

Department of Psychology, University of Vermont, Burlington 05405, USA.

出版信息

Behav Neurosci. 2000 Oct;114(5):998-1004. doi: 10.1037//0735-7044.114.5.998.

DOI:10.1037//0735-7044.114.5.998

PMID:11085615

Abstract

Fear-potentiated startle was assessed in mice with a targeted disruption of the alpha and delta isoforms of the transcription factor cAMP response element binding protein (CREB) 24 hr after 5 tone + shock training trials. Whereas wild-type mice showed fear-potentiated startle that persisted up to 45 days after training, CREBalphadelta-/- mice failed to show fear-potentiated startle. However, CREBalphadelta-/- and wild-type mice had similar startle amplitudes and similar magnitudes of prepulse inhibition of startle, suggesting that CREBalphadelta-/- mice have no obvious sensory or motor deficits. These results add to the literature indicating that CREB-activated transcription plays a critical role in the formation of long-term memory and illustrate the utility of the fear-potentiated startle paradigm for assessing cognition in genetically altered mice.

摘要

在经过5次音调+电击训练试验24小时后，对转录因子环磷腺苷反应元件结合蛋白（CREB）的α和δ亚型发生靶向破坏的小鼠进行了恐惧增强惊吓反应评估。野生型小鼠表现出恐惧增强惊吓反应，该反应在训练后持续长达45天，而CREBαδ基因敲除小鼠未能表现出恐惧增强惊吓反应。然而，CREBαδ基因敲除小鼠和野生型小鼠具有相似的惊吓幅度以及相似的惊吓前脉冲抑制程度，这表明CREBαδ基因敲除小鼠没有明显的感觉或运动缺陷。这些结果补充了相关文献，表明CREB激活的转录在长期记忆形成中起关键作用，并说明了恐惧增强惊吓范式在评估基因改变小鼠认知方面的效用。