Winkler Maria Teresa, Schang Luis S, Doster Alan, Holt Todd, Jones Clinton
Department of Veterinary and Biomedical Sciences, Center for Biotechnology, University of Nebraska, Fair Street at East Campus Loop, Lincoln, NE 68583-0905, USA1.
J Gen Virol. 2000 Dec;81(Pt 12):2993-2998. doi: 10.1099/0022-1317-81-12-2993.
Following acute infection of cattle with bovine herpesvirus-1 (BHV-1), cyclin expression was examined in trigeminal ganglia (TG). Cyclin A was primarily detected in the nucleus of TG neurons. In contrast, cyclin D1 and cyclin E were detected in the nucleus and cytoplasm of TG neurons. Uninfected or latently infected calves did not express detectable levels of these cyclins in TG neurons. Following dexamethasone-induced reactivation, cyclins D1, E and A were also detected in TG neurons. In situ hybridization of consecutive sections demonstrated that many neurons expressing cyclins contained viral nucleic acid, demonstrating that they were infected. Based on these observations, we hypothesize that BHV-1 infection activates neuronal cyclin expression to enhance productive infection. It is also possible that the stress of neuronal infection or reactivation leads to cyclin expression.
在用牛疱疹病毒1型（BHV-1）对牛进行急性感染后，检测了三叉神经节（TG）中的细胞周期蛋白表达。细胞周期蛋白A主要在TG神经元的细胞核中检测到。相比之下，细胞周期蛋白D1和细胞周期蛋白E在TG神经元的细胞核和细胞质中均有检测到。未感染或潜伏感染的小牛在TG神经元中未表达可检测水平的这些细胞周期蛋白。在用地塞米松诱导再激活后，TG神经元中也检测到了细胞周期蛋白D1、E和A。连续切片的原位杂交表明，许多表达细胞周期蛋白的神经元含有病毒核酸，表明它们被感染了。基于这些观察结果，我们推测BHV-1感染激活神经元细胞周期蛋白表达以增强增殖性感染。神经元感染或再激活的应激也可能导致细胞周期蛋白表达。
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