Shi J, Townsend M, Constantine-Paton M
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Neuron. 2000 Oct;28(1):103-14. doi: 10.1016/s0896-6273(00)00089-1.
Whole-cell recording in the superficial layers of the developing superior colliculus (sSC) reveals a large drop in NMDA receptor (NMDAR) current decay time synchronized across all neurons and occurring consistently between P10 and P11. We show that blocking the Ca2+/calmodulin-dependent phosphatase calcineurin (CaN) in the postsynaptic neuron can abolish this drop. The regulation is induced prematurely by 1-2 hr of electrical stimulation in P10 collicular slices only if CaN and NMDAR currents can be activated in the neuron. These data suggest that a long-lasting, CaN-mediated control of NMDAR kinetics is rapidly initiated by heightened activity of the NMDAR itself and demonstrate a novel developmental and tonic function of CaN that can play an important role in modulating the plasticity of the developing CNS.
在发育中的上丘浅层（sSC）进行全细胞记录发现，NMDA受体（NMDAR）电流衰减时间大幅下降，所有神经元同步出现，且在出生后第10天（P10）和第11天（P11）之间持续发生。我们发现，阻断突触后神经元中Ca2+/钙调蛋白依赖性磷酸酶钙调神经磷酸酶（CaN）可消除这种下降。仅当神经元中CaN和NMDAR电流能够被激活时，在P10丘脑中进行1-2小时的电刺激会过早诱导这种调节。这些数据表明，NMDAR自身活性增强会迅速启动由CaN介导的对NMDAR动力学的持久控制，并证明了CaN具有一种新的发育和紧张性功能，其在调节发育中的中枢神经系统可塑性方面可能发挥重要作用。
