Fear M W, Kelsell D P, Spurr N K, Barnes M R
Centre for Cutaneous Research, St. Bartholomew's and the Royal London Hospital School of Medicine and Dentistry, 2 Newark Street, London, E1 2AT, United Kingdom.
Biochem Biophys Res Commun. 2000 Nov 30;278(3):814-20. doi: 10.1006/bbrc.2000.3852.
The WNT genes encode a large family of secreted glycoprotein signalling molecules important from the earliest stages of development through to the adult. We have identified a novel isoform of the recently described WNT family member, Wnt16, following analysis of chromosome 7q31 genomic sequence. We find differential organisation of Wnt16 with the generation of two mRNA isoforms, Wnt16a and Wnt16b. These isoforms differ in the composition of their 5'-UTR and first exons and show evidence of differential expression. In normal human tissues, Wnt16a is expressed at significant levels only in the pancreas, whereas Wnt16b is expressed more ubiquitously with highest levels in adult kidney, placenta, brain, heart, and spleen. Wnt16 is one of a growing number of WNT genes showing evidence of distinct isoforms. We present evidence to suggest that these isoforms may be regulated from alternative promoters and discuss the potential functional differentiation afforded by these WNT isoforms. This may reveal subtle new mechanisms of regulation of WNT expression and function.
WNT基因编码一大类分泌型糖蛋白信号分子，从发育的最早阶段到成年期都很重要。在对7号染色体q31基因组序列进行分析后，我们鉴定出了最近描述的WNT家族成员Wnt16的一种新型异构体。我们发现Wnt16存在差异组织，产生了两种mRNA异构体Wnt16a和Wnt16b。这些异构体在其5'-UTR和第一个外显子的组成上有所不同，并显示出差异表达的证据。在正常人体组织中，Wnt16a仅在胰腺中大量表达，而Wnt16b表达更为广泛，在成年肾脏、胎盘、大脑、心脏和脾脏中表达水平最高。Wnt16是越来越多显示出不同异构体证据的WNT基因之一。我们提供的证据表明，这些异构体可能由不同的启动子调控，并讨论了这些WNT异构体提供的潜在功能分化。这可能揭示WNT表达和功能调控的微妙新机制。
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