4期神经母细胞瘤中经激光捕获显微切割的雪旺细胞和成神经细胞具有相同的基因改变。
Laser-capture microdissected schwannian and neuroblastic cells in stage 4 neuroblastomas have the same genetic alterations.
作者信息
Mora J, Akram M, Cheung N K, Chen L, Gerald W L
机构信息
Department of Pediatrics, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
出版信息
Med Pediatr Oncol. 2000 Dec;35(6):534-7. doi: 10.1002/1096-911x(20001201)35:6<534::aid-mpo7>3.0.co;2-r.
BACKGROUND
Neuroblastoma (NB) is a heterogeneous tumor composed of mixed populations of neuroblastic, Schwannian, and gangliocytic cells.
PROCEDURE
We examined the genetic lineage of Schwannian and neuroblastic cells in stage 4 NB using laser-capture microdissection (LCM) and allelic analysis of microsatellite markers of chromosomes 1p, 11q, and 14q.
RESULTS
In 18 out of 19 cases, individual cell types exhibited the same allelotype.
CONCLUSION
Our results provide evidence that the majority of Schwannian and neuroblastic cells in stage 4 NB are of neoplastic origin, and support the hypothesis of a tumor stem cell capable of development along multiple lineages.
背景
神经母细胞瘤（NB）是一种由神经母细胞、施万细胞和神经节细胞混合群体组成的异质性肿瘤。
方法
我们使用激光捕获显微切割（LCM）和对1号染色体、11号染色体和14号染色体微卫星标记的等位基因分析，研究了4期NB中施万细胞和神经母细胞的遗传谱系。
结果
在19例中的18例中，单个细胞类型表现出相同的等位基因型。
结论
我们的结果提供了证据，表明4期NB中的大多数施万细胞和神经母细胞起源于肿瘤，并支持肿瘤干细胞能够沿多个谱系发育的假说。
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