Kim C H, Bak K H, Kim Y S, Kim J M, Ko Y, Oh S J, Kim K M, Hong E K
Department of Neurosurgery, College of Medicine, Hanyang University, Seoul, South Korea.
Surg Neurol. 2000 Sep;54(3):235-40. doi: 10.1016/s0090-3019(00)00307-4.
The expression and distribution of the extracellular matrix protein tenascin-C (TN-C) may be enhanced in human astrocytomas. The purpose of this study is to evaluate the expression of TN-C according to histological malignancy of tumor cells and its relevance to neoplastic angiogenesis in human astrocytic tumors.
Between 1994 and 1998, 52 astrocytic tumor specimens including 4 pilocytic astrocytomas, 13 astrocytomas, 3 anaplastic astrocytomas, and 32 glioblastomas were used in this study. A retrospective analysis was performed to evaluate a statistical correlation between TN-C expression and proliferative indices. We characterized the expression of TN-C in neoplastic vessels, around individual tumor cells as a tumor network, and in tumor cells by immunohistochemistry using antibodies against human TN-C. The proliferative indices were also investigated by immunostaining with the MIB-1 antibody against the Ki-67 proliferation antigen.
TN-C immunoreactivity was found to be enhanced in tumor vessels and tumor networks of high-grade astrocytic tumors. The vascular TN-C deposition was greater in high-grade than in low-grade astrocytic tumors (p < 0.05). Its expression was the most intense in glioblastomas. Proliferation indices increased with tumor grade and MIB-1 labeling index (LI) was highest in glioblastomas. Moreover, expression of TN-C in tumor vessels was correlated with proliferative indices.
Our data show that TN-C in human astrocytic tumors may be identified as a factor contributing to malignant progression. And also, enhanced expression of TN-C in tumor vessels having a high proliferative index indicates that TN-C could be involved in neoplastic angiogenesis.
细胞外基质蛋白肌腱蛋白-C（TN-C）在人类星形细胞瘤中的表达和分布可能会增强。本研究的目的是根据肿瘤细胞的组织学恶性程度评估TN-C的表达及其与人类星形细胞肿瘤中肿瘤血管生成的相关性。
在1994年至1998年期间，本研究使用了52个星形细胞肿瘤标本，包括4个毛细胞型星形细胞瘤、13个星形细胞瘤、3个间变性星形细胞瘤和32个胶质母细胞瘤。进行回顾性分析以评估TN-C表达与增殖指数之间的统计相关性。我们通过使用抗人TN-C抗体的免疫组织化学方法，对肿瘤血管、作为肿瘤网络的单个肿瘤细胞周围以及肿瘤细胞中TN-C的表达进行了表征。还通过用抗Ki-67增殖抗原的MIB-1抗体进行免疫染色来研究增殖指数。
发现TN-C免疫反应性在高级别星形细胞肿瘤的肿瘤血管和肿瘤网络中增强。高级别星形细胞肿瘤中血管TN-C沉积比低级别星形细胞肿瘤更大（p < 0.05）。其表达在胶质母细胞瘤中最为强烈。增殖指数随肿瘤级别增加，MIB-1标记指数（LI）在胶质母细胞瘤中最高。此外，肿瘤血管中TN-C的表达与增殖指数相关。
我们的数据表明，人类星形细胞肿瘤中的TN-C可能被确定为促成恶性进展的一个因素。而且，TN-C在具有高增殖指数的肿瘤血管中的表达增强表明TN-C可能参与肿瘤血管生成。
