Jackson R C
J Biol Chem. 1975 Jan 25;250(2):617-22.
The exterior surface of mature chicken erythrocytes has been labeled with a cationic membrane impermeable reagent, rho-nitrophenyl-N,N,N-trimethy[125I]iodotyrosinate. This reagent forms stable covalent bonds with the amino groups of exposed protein and lipid companents. Two major protein components with subunit molecular weights of 100,000 and 55,000 are found on the outer surface of mature chicken erythrocytes. Both of the labeled components coincide were periodic acid-Schiff base-stained areas on sodium dodecyl sulfate polyacrylamide gel electrophoresis indicating that they may be glycoproteins.
成熟鸡红细胞的外表面已用一种阳离子性膜不可渗透试剂——ρ-硝基苯基-N,N,N-三甲基[¹²⁵I]碘酪氨酸酯进行标记。该试剂与暴露的蛋白质和脂质成分的氨基形成稳定的共价键。在成熟鸡红细胞的外表面发现了两种主要的蛋白质成分,其亚基分子量分别为100,000和55,000。在十二烷基硫酸钠聚丙烯酰胺凝胶电泳上,这两种标记成分都与高碘酸-席夫碱染色区域重合,表明它们可能是糖蛋白。