Schick B, Berke G
Transplantation. 1979 Jun;27(6):365-8.
A method to determine directly the antigenic similarity of target cells (TCs) involved in cytotoxic T lymphocyte (CTL)-TC interactions is described. When fluorescently labeled TC (FL-TCs) are bound (conjugated) to CTLs in the presence of antigenically similar but unlabeled TCs, the percentage of CTL-TC conjugates containing unlabeled TCs corresponds linearly to the number of unlabeled TCs in the TC population. This assay is specific, permits direct observation of individual CTLs and TCs which interact to form conjugates, can be completed within 10 min, eliminates complications attributable to CTL recycling, and the degree of competition observed fits that theoretically expected. The results of this paper provide the theoretical basis for "cold" (unlabeled) target inhibition of radioactive assays of lymphocyte-mediated cytolysis.
本文描述了一种直接测定参与细胞毒性T淋巴细胞（CTL）-靶细胞（TC）相互作用的靶细胞（TC）抗原相似性的方法。当荧光标记的靶细胞（FL-TC）在存在抗原相似但未标记的靶细胞的情况下与CTL结合（共轭）时，含有未标记靶细胞的CTL-TC共轭物的百分比与靶细胞群体中未标记靶细胞的数量呈线性对应。该测定具有特异性，允许直接观察相互作用形成共轭物的单个CTL和TC，可在10分钟内完成，消除了因CTL循环导致的并发症，并且观察到的竞争程度符合理论预期。本文的结果为淋巴细胞介导的细胞溶解放射性测定的“冷”（未标记）靶标抑制提供了理论基础。
