Chernaeva L
Institute of Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Acta Physiol Pharmacol Bulg. 2000;25(1):27-31.
The mediators of non-adrenergic non-cholinergic (NANC) relaxation of cat gastric fundus were examined in vitro. Electrical field stimulation (EFS, 10 pulses train and 10 s train) induced tetrodotoxin (TTX) sensitive relaxations in the presence of atropine and guanethidine. Relaxation induced by vasoactive intestinal polypeptide (VIP) was abolished by alpha-chymotrypsin (alpha-CT). N omega-nitro-L-arginine (L-NNA) completely inhibited relaxation induced by EFS of 10 pulses train at all frequencies. L-NNA completely inhibited the relaxation induced by EFS of 10 s train at low frequencies but partly that at high frequencies; alpha-CT had an additive to L-NNA inhibitory effect on the relaxation. The results suggest a nitrergic nature of NANC relaxation in cat gastric fundus upon EFS of 10 pulses, and a nitrergic and peptidergic (VIP) nature of relaxations upon EFS of 10 s train.
在体外研究了猫胃底非肾上腺素能非胆碱能（NANC）舒张的介质。在阿托品和胍乙啶存在的情况下，电场刺激（EFS，10个脉冲串和10秒串）诱导了对河豚毒素（TTX）敏感的舒张。血管活性肠肽（VIP）诱导的舒张被α-糜蛋白酶（α-CT）消除。Nω-硝基-L-精氨酸（L-NNA）在所有频率下均完全抑制10个脉冲串的EFS诱导的舒张。L-NNA在低频时完全抑制10秒串的EFS诱导的舒张，但在高频时仅部分抑制；α-CT对L-NNA抑制舒张的作用具有相加效应。结果表明，10个脉冲的EFS作用下猫胃底NANC舒张具有一氧化氮能性质，而10秒串的EFS作用下舒张具有一氧化氮能和肽能（VIP）性质。
