长春新碱对Balb/c雌性小鼠体内99m锝标记的二乙三胺五乙酸（DTPA）、葡庚糖酸钙（GHA）和二巯基丁二酸（DMSA）生物分布的影响。

The effect of vincristine on the biodistribution of technetium-99m DTPA, GHA, and DMSA in Balb/c female mice.

作者信息

Mattos D M, Gomes M L, Freitas R S, Boasquevisque E M, Cardoso V N, Paula E F, Bernardo-Filho M

机构信息

Instituto Nacional de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Brasil.

出版信息

J Nucl Med Technol. 2000 Dec;28(4):271-4.

PMID:11142330

Abstract

OBJECTIVE

Vincristine has been widely used in various chemotherapeutic protocols in oncology. The purpose of this study was to evaluate the effect of vincristine on the biodistribution of 99mTc-DMSA, 99mTc-GHA, and 99mTc-DTPA in Balb/c female mice.

METHODS

Vincristine (0.03 mg, 0.3 mL) was injected into female isogenic Balb/c mice (n = 15), in 3 doses over an interval of 96 h. The 99mTc-DMSA, 99mTc-GHA, or 99mTc-DTPA (7.4 MBq) was administered after the last dose of vincristine. After 0.5 h the animals were killed rapidly. The organs (pancreas, thyroid, brain, thymus, ovary, uterus, spleen, kidney, heart, stomach, lung, liver, bone, and lymph nodes) were isolated and the radioactivity in each organ was counted in a NaI(Tl) well counter. The percentage of radioactivity (%) in each was calculated and compared with the control group. Statistical analysis was performed by Wilcoxon test (P < 0.05).

RESULTS

The percentage of 99mTc-DMSA was increased in the lung, pancreas, heart, thyroid, brain, bone, and lymph nodes (inguinal and mesenteric). The percentage of 99mTc-GHA was decreased in the uterus, ovary, spleen, thymus, lymph nodes (inguinal and mesenteric), kidney, and heart. The percentage of 99mTc-DTPAwas increased in thymus, lymph nodes (inguinal and mesenteric), ovary, uterus, spleen, kidney, heart, stomach, lung, liver, and bone.

CONCLUSION

The results could be explained by the metabolization, toxic effect, therapeutic, or immunosupressive action of the studied chemotherapeutic drug.

摘要

目的

长春新碱已广泛应用于肿瘤学的各种化疗方案中。本研究的目的是评估长春新碱对99mTc - DMSA、99mTc - GHA和99mTc - DTPA在Balb/c雌性小鼠体内生物分布的影响。

方法

将长春新碱（0.03 mg，0.3 mL）分3剂在96小时的间隔内注射到同基因雌性Balb/c小鼠（n = 15）体内。在最后一剂长春新碱后给予99mTc - DMSA、99mTc - GHA或99mTc - DTPA（7.4 MBq）。0.5小时后迅速处死动物。分离出器官（胰腺、甲状腺、脑、胸腺、卵巢、子宫、脾脏、肾脏、心脏、胃、肺、肝脏、骨骼和淋巴结），并在NaI（Tl）井型计数器中对每个器官的放射性进行计数。计算每个器官中放射性的百分比（%），并与对照组进行比较。采用Wilcoxon检验进行统计学分析（P < 0.05）。

结果

99mTc - DMSA在肺、胰腺、心脏、甲状腺、脑、骨骼和淋巴结（腹股沟和肠系膜）中的百分比增加。99mTc - GHA在子宫、卵巢、脾脏、胸腺、淋巴结（腹股沟和肠系膜）、肾脏和心脏中的百分比降低。99mTc - DTPA在胸腺、淋巴结（腹股沟和肠系膜）、卵巢、子宫、脾脏、肾脏、心脏、胃、肺、肝脏和骨骼中的百分比增加。