Castán P, de Pedro M A, Risco C, Vallés C, Fernández L A, Schwarz H, Berenguer J
Departamento and Centro de Biología Molecular Severo Ochoa, CSIC-UAM, CSIC Campus de Cantoblanco, Madrid 28049, Spain.
J Bacteriol. 2001 Feb;183(4):1491-4. doi: 10.1128/JB.183.4.1491-1494.2001.
The role of the 5' untranslated region (5'UTR) of the S-layer gene from Thermus thermophilus was analyzed through the isolation of Delta 5'UTR mutants. In these mutants the half-life of splA mRNA was strongly reduced and slpA transcription was no longer subjected to growth phase-dependent repression. Overproduction and detachment of the external envelopes of the mutants were observed in stationary phase.
通过分离Δ5'UTR突变体,分析了嗜热栖热菌S层基因5'非翻译区(5'UTR)的作用。在这些突变体中,splA mRNA的半衰期大幅缩短,且slpA转录不再受生长阶段依赖性抑制。在稳定期观察到突变体外部包膜的过量产生和脱离。