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百合科和芦荟科ndhD的初级转录本需要对起始密码子和第20个密码子进行编辑。

Primary transcripts of ndhD of Liliaceae and Aloaceae require editing of the start and 20th codons.

作者信息

López-Serrano M, Del Campo E M, Sabater B, Martín M

机构信息

Departamento de Biología Vegetal (Fisiología Vegetal), Universidad de Alcalá, Alcalá de Henares, 28871-Madrid, Spain.

出版信息

J Exp Bot. 2001 Jan;52(354):179-80.

Abstract

About 350 nucleotide sequences around the start codon of the plastid ndhD gene were determined in six species to investigate the requirement of C to U editing of the cryptic start codons. The comparison with other sequences in databanks showed that, in contrast to grasses and similarly to dicots, Liliaceae and Aloaceae require editing of the primary transcript to generate the AUG start codon. Primary transcripts of Liliaceae and Aloaceae also show a new editing site at the 59th nucleotide, which has not been described in other plants. In some cases, transcripts showed partial editing which suggests that the synthesis of the protein may be controlled at the level of transcript processing.

摘要

测定了六个物种中质体ndhD基因起始密码子周围约350个核苷酸序列,以研究隐蔽起始密码子C到U编辑的需求。与数据库中的其他序列比较表明,与禾本科植物不同,与双子叶植物类似,百合科和芦荟科需要对初级转录本进行编辑以产生AUG起始密码子。百合科和芦荟科的初级转录本在第59个核苷酸处还显示出一个新的编辑位点,这在其他植物中尚未有描述。在某些情况下,转录本显示出部分编辑,这表明蛋白质的合成可能在转录本加工水平上受到控制。

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